In the PAS diastase digestion protocol, what is observed for glycogen in the section treated with diastase?

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Multiple Choice

In the PAS diastase digestion protocol, what is observed for glycogen in the section treated with diastase?

Explanation:
Diastase digests glycogen, so when the tissue is treated with diastase before the PAS stain, the glycogen is removed and no magenta signal for glycogen remains. The section will show glycogen absent, distinguishing glycogen-containing areas from other PAS‑positive substances that resist diastase. This is why “absent” is the correct observation.

Diastase digests glycogen, so when the tissue is treated with diastase before the PAS stain, the glycogen is removed and no magenta signal for glycogen remains. The section will show glycogen absent, distinguishing glycogen-containing areas from other PAS‑positive substances that resist diastase. This is why “absent” is the correct observation.

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