In the Gridley procedure, the aldehyde fuchsin stain will attach to which reagent?

Prepare for the Histotechnologist Certification Exam with our comprehensive study material. Use flashcards, detailed explanations, and intuitive multiple-choice questions. Boost your test readiness and achieve certification success!

Multiple Choice

In the Gridley procedure, the aldehyde fuchsin stain will attach to which reagent?

Explanation:
Aldehyde fuchsin relies on a reaction with Schiff reagent. Schiff reagent is designed to react with aldehyde groups to produce a magenta color. In the Gridley procedure, the aldehyde groups in aldehyde fuchsin form a Schiff base with Schiff reagent, so the dye effectively attaches to Schiff reagent and becomes visibly colored. The other reagents listed do not participate in this specific aldehyde–Schiff interaction: chromic acid is an oxidizer/bleaching step, light green is a counterstain, and ninhydrin reacts with amino acids.

Aldehyde fuchsin relies on a reaction with Schiff reagent. Schiff reagent is designed to react with aldehyde groups to produce a magenta color. In the Gridley procedure, the aldehyde groups in aldehyde fuchsin form a Schiff base with Schiff reagent, so the dye effectively attaches to Schiff reagent and becomes visibly colored. The other reagents listed do not participate in this specific aldehyde–Schiff interaction: chromic acid is an oxidizer/bleaching step, light green is a counterstain, and ninhydrin reacts with amino acids.

More Multiple Choice Questions
Subscribe

Get the latest from Passetra

You can unsubscribe at any time. Read our privacy policy