In the dehydration steps, which concentration is used first?

Prepare for the Histotechnologist Certification Exam with our comprehensive study material. Use flashcards, detailed explanations, and intuitive multiple-choice questions. Boost your test readiness and achieve certification success!

Multiple Choice

In the dehydration steps, which concentration is used first?

Explanation:
Dehydration in histology is done by gradually replacing water in the tissue with ethanol using increasing concentrations. Starting with the lowest ethanol concentration lets water diffuse out gently, reducing osmotic shock and preserving tissue structure. After that, you progressively use higher concentrations to complete the dehydration before clearing. Among the options, the lowest concentration is 65% ethanol, so it’s used first. You would then move to higher concentrations (for example, 70%, then 95%), ending with absolute ethanol. Using a very high concentration at the start can cause rapid dehydration and artefacts.

Dehydration in histology is done by gradually replacing water in the tissue with ethanol using increasing concentrations. Starting with the lowest ethanol concentration lets water diffuse out gently, reducing osmotic shock and preserving tissue structure. After that, you progressively use higher concentrations to complete the dehydration before clearing. Among the options, the lowest concentration is 65% ethanol, so it’s used first. You would then move to higher concentrations (for example, 70%, then 95%), ending with absolute ethanol. Using a very high concentration at the start can cause rapid dehydration and artefacts.

More Multiple Choice Questions
Subscribe

Get the latest from Passetra

You can unsubscribe at any time. Read our privacy policy