In the Brown-Hopps modification of the Gram stain for tissues, Gram-positive organisms appear as which color?

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Multiple Choice

In the Brown-Hopps modification of the Gram stain for tissues, Gram-positive organisms appear as which color?

Explanation:
In the Brown-Hopps modification for tissue Gram staining, the thick peptidoglycan layer of Gram-positive bacteria traps the crystal violet-iodine complex and resists decolorization. This causes them to retain the dark dye and appear blue-black under the microscope. Gram-negative bacteria, with thinner walls, lose the primary stain during decolorization and take up the counterstain, showing pink. So the color seen for Gram-positive organisms with this method is blue-black.

In the Brown-Hopps modification for tissue Gram staining, the thick peptidoglycan layer of Gram-positive bacteria traps the crystal violet-iodine complex and resists decolorization. This causes them to retain the dark dye and appear blue-black under the microscope. Gram-negative bacteria, with thinner walls, lose the primary stain during decolorization and take up the counterstain, showing pink. So the color seen for Gram-positive organisms with this method is blue-black.

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