In the Brown and Brenn Gram stain, over-differentiation with picric acid/acetone causes nuclei to appear which color?

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Multiple Choice

In the Brown and Brenn Gram stain, over-differentiation with picric acid/acetone causes nuclei to appear which color?

Explanation:
In this staining method, the differentiating step uses picric acid in acetone to decolorize. If that step is taken too far, it strips away the primary stain and introduces a strong yellow from the picric acid itself. This excess decolorization can cause the nuclear material in tissue to take on that yellow hue, so the nuclei appear yellow.

In this staining method, the differentiating step uses picric acid in acetone to decolorize. If that step is taken too far, it strips away the primary stain and introduces a strong yellow from the picric acid itself. This excess decolorization can cause the nuclear material in tissue to take on that yellow hue, so the nuclei appear yellow.

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