In Southgate's Mucicarmine technique, what are the results for glycogen, neutral mucin, and acid mucin?

Prepare for the Histotechnologist Certification Exam with our comprehensive study material. Use flashcards, detailed explanations, and intuitive multiple-choice questions. Boost your test readiness and achieve certification success!

Multiple Choice

In Southgate's Mucicarmine technique, what are the results for glycogen, neutral mucin, and acid mucin?

Explanation:
In this technique, mucicarmine specifically stains acidic mucins magenta, while neutral mucins and glycogen are not stained by the mucicarmine stain used in Southgate’s method. Acid mucins contain sulfated or carboxylated mucopolysaccharides that bind the dye, giving a magenta color. Neutral mucins lack these groups, so they remain unstained, and glycogen isn’t targeted by this stain at all, so it also remains negative. Therefore the expected pattern is glycogen negative, neutral mucin negative, acid mucin positive.

In this technique, mucicarmine specifically stains acidic mucins magenta, while neutral mucins and glycogen are not stained by the mucicarmine stain used in Southgate’s method. Acid mucins contain sulfated or carboxylated mucopolysaccharides that bind the dye, giving a magenta color. Neutral mucins lack these groups, so they remain unstained, and glycogen isn’t targeted by this stain at all, so it also remains negative. Therefore the expected pattern is glycogen negative, neutral mucin negative, acid mucin positive.

More Multiple Choice Questions
Subscribe

Get the latest from Passetra

You can unsubscribe at any time. Read our privacy policy