In PAS staining, what are the expected results for glycogen, neutral mucin, and acid mucin?

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Multiple Choice

In PAS staining, what are the expected results for glycogen, neutral mucin, and acid mucin?

Explanation:
Periodic acid–Schiff staining detects carbohydrates with vicinal diols, so it highlights glycogen and neutral mucins, which have such carbohydrate moieties. Glycogen is a storage polysaccharide rich in diols, so it stains positive. Neutral mucins are glycoproteins with neutral sugar chains, also producing a positive Schiff reaction. Acid mucins, which contain sulfated or sialylated sugars, do not react with the Schiff reagent in PAS and thus remain negative. Therefore, glycogen positive, neutral mucin positive, and acid mucin negative.

Periodic acid–Schiff staining detects carbohydrates with vicinal diols, so it highlights glycogen and neutral mucins, which have such carbohydrate moieties. Glycogen is a storage polysaccharide rich in diols, so it stains positive. Neutral mucins are glycoproteins with neutral sugar chains, also producing a positive Schiff reaction. Acid mucins, which contain sulfated or sialylated sugars, do not react with the Schiff reagent in PAS and thus remain negative. Therefore, glycogen positive, neutral mucin positive, and acid mucin negative.

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