In PAS staining after diastase digestion, what is the background color when glycogen is removed?

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Multiple Choice

In PAS staining after diastase digestion, what is the background color when glycogen is removed?

Explanation:
PAS staining detects carbohydrate-rich structures by forming a magenta color with Schiff reagent. When diastase is used before staining, it digests and removes glycogen, so glycogen-containing areas no longer develop the magenta signal. What remains magenta are non-glycogen carbohydrates, such as mucopolysaccharides, while the rest of the tissue that does not react with Schiff ends up with a light, pink background from the stain’s background/counterstain. So the background after diastase digestion is pale pink.

PAS staining detects carbohydrate-rich structures by forming a magenta color with Schiff reagent. When diastase is used before staining, it digests and removes glycogen, so glycogen-containing areas no longer develop the magenta signal. What remains magenta are non-glycogen carbohydrates, such as mucopolysaccharides, while the rest of the tissue that does not react with Schiff ends up with a light, pink background from the stain’s background/counterstain. So the background after diastase digestion is pale pink.

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