In PAS staining after diastase digestion, the background color is pale pink.

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Multiple Choice

In PAS staining after diastase digestion, the background color is pale pink.

Explanation:
In PAS staining, carbohydrates react with Schiff reagent to give a magenta color. When diastase digestion is used, glycogen—the major PAS‑positive carbohydrate—is broken down and removed from the tissue. This reduces the amount of background PAS reaction, so most of the tissue lacks magenta and instead takes up the counterstain, appearing pale pink. Only diastase‑resistant carbohydrate structures (like certain mucopolysaccharides) remain magenta. So the pale pink background after diastase digestion reflects the loss of glycogen and the counterstain filling in the rest of the tissue.

In PAS staining, carbohydrates react with Schiff reagent to give a magenta color. When diastase digestion is used, glycogen—the major PAS‑positive carbohydrate—is broken down and removed from the tissue. This reduces the amount of background PAS reaction, so most of the tissue lacks magenta and instead takes up the counterstain, appearing pale pink. Only diastase‑resistant carbohydrate structures (like certain mucopolysaccharides) remain magenta. So the pale pink background after diastase digestion reflects the loss of glycogen and the counterstain filling in the rest of the tissue.

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