In Methyl Green-Pyronin Y staining, which component stains DNA?

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Multiple Choice

In Methyl Green-Pyronin Y staining, which component stains DNA?

Explanation:
Methyl Green is the stain that binds DNA. It has a strong affinity for the DNA phosphate backbone, producing the green color seen in DNA-rich areas like nuclei. Pyronin Y, the counterstain, binds RNA and colors RNA-containing structures pink/red, providing contrast to the green-stained DNA. The acetate buffer simply creates the right chemical environment (pH and ionic strength) for staining and does not color the sample. Glycogen is not stained by this combination.

Methyl Green is the stain that binds DNA. It has a strong affinity for the DNA phosphate backbone, producing the green color seen in DNA-rich areas like nuclei. Pyronin Y, the counterstain, binds RNA and colors RNA-containing structures pink/red, providing contrast to the green-stained DNA. The acetate buffer simply creates the right chemical environment (pH and ionic strength) for staining and does not color the sample. Glycogen is not stained by this combination.

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