In Masson's Trichrome staining, what color is collagen?

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Multiple Choice

In Masson's Trichrome staining, what color is collagen?

Explanation:
Masson's Trichrome differentiates tissue components by color, with collagen uniquely binding the blue dye used in the stain. Depending on the exact dye formulation, that blue dye can read as a pure blue or appear greenish when a green counterstain is involved, giving collagen a blue–green appearance. This color distinction helps separate connective tissue from muscle and cytoplasm (which stain red/pink) and from nuclei (which stay dark). So collagen appearing blue or blue-green is expected and is the hallmark of this stain’s differentiation.

Masson's Trichrome differentiates tissue components by color, with collagen uniquely binding the blue dye used in the stain. Depending on the exact dye formulation, that blue dye can read as a pure blue or appear greenish when a green counterstain is involved, giving collagen a blue–green appearance. This color distinction helps separate connective tissue from muscle and cytoplasm (which stain red/pink) and from nuclei (which stay dark). So collagen appearing blue or blue-green is expected and is the hallmark of this stain’s differentiation.

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