In hematoxylin and eosin staining, if tissue is over-differentiated with acid alcohol, the cytoplasm appears what color?

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Multiple Choice

In hematoxylin and eosin staining, if tissue is over-differentiated with acid alcohol, the cytoplasm appears what color?

Explanation:
In hematoxylin and eosin staining, the cytoplasm takes on pink from eosin, while nuclei are blue/purple from hematoxylin. The differentiation step with acid alcohol removes excess hematoxylin to prevent overstaining of nuclei. If the tissue is over-differentiated, too much hematoxylin is removed, causing nuclear color to fade, but eosin staining of the cytoplasm remains, so the cytoplasm still appears pink.

In hematoxylin and eosin staining, the cytoplasm takes on pink from eosin, while nuclei are blue/purple from hematoxylin. The differentiation step with acid alcohol removes excess hematoxylin to prevent overstaining of nuclei. If the tissue is over-differentiated, too much hematoxylin is removed, causing nuclear color to fade, but eosin staining of the cytoplasm remains, so the cytoplasm still appears pink.

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