In Alcian Blue-PAS-Hematoxylin, how do acidic mucosubstances appear?

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Multiple Choice

In Alcian Blue-PAS-Hematoxylin, how do acidic mucosubstances appear?

Explanation:
Acidic mucosubstances appear blue because Alcian Blue is a positively charged dye that binds specifically to the negatively charged groups (sulfate and carboxyl) in acidic mucopolysaccharides. In the Alcian Blue-PAS-Hematoxylin sequence, this blue staining marks the acidic components, while the PAS step colors neutral mucosubstances magenta, and Hematoxylin provides nuclear contrast. So the acidic mucins show a blue color, contrasting with the pink-magenta neutral mucins.

Acidic mucosubstances appear blue because Alcian Blue is a positively charged dye that binds specifically to the negatively charged groups (sulfate and carboxyl) in acidic mucopolysaccharides. In the Alcian Blue-PAS-Hematoxylin sequence, this blue staining marks the acidic components, while the PAS step colors neutral mucosubstances magenta, and Hematoxylin provides nuclear contrast. So the acidic mucins show a blue color, contrasting with the pink-magenta neutral mucins.

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