If adjacent PAS sections are stained with and without diastase, and the section without digestion is positive while the one with digestion is negative, what does this indicate?

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Multiple Choice

If adjacent PAS sections are stained with and without diastase, and the section without digestion is positive while the one with digestion is negative, what does this indicate?

PAS detects carbohydrate-rich substances, including glycogen, mucin, and basement membranes. When a section is pretreated with diastase, glycogen is digested and loses its PAS positivity, while diastase-resistant carbohydrates (like most mucins and basement membrane components) remain PAS-positive. So, if a non-digested section is positive and the diastase-digested section becomes negative, the PAS staining came from glycogen. The result identifies glycogen as the substance responsible for the staining.

If adjacent PAS sections are stained with and without diastase, and the section without digestion is positive while the one with digestion is negative, what does this indicate?

Prepare for the Histotechnologist Certification Exam with our comprehensive study material. Use flashcards, detailed explanations, and intuitive multiple-choice questions. Boost your test readiness and achieve certification success!

If adjacent PAS sections are stained with and without diastase, and the section without digestion is positive while the one with digestion is negative, what does this indicate?

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