Hotchkiss-McManus PAS Reaction is used to demonstrate which type of organism?

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Multiple Choice

Hotchkiss-McManus PAS Reaction is used to demonstrate which type of organism?

Explanation:
Periodic acid–Schiff (PAS) reactions highlight carbohydrate-rich structures, turning them magenta after oxidation and Schiff reagent treatment. Fungal cell walls contain abundant polysaccharides such as chitin and glucans, which react strongly with PAS. The Hotchkiss-McManus modification of this stain is used in histology to visualize fungal elements in fixed tissue sections, making fungi the best target for demonstration with this reaction. Bacteria, nuclei, and spores don’t rely on the same polysaccharide-rich cell-wall features that yield a strong PAS signal in this context, so they are not the primary targets of this technique.

Periodic acid–Schiff (PAS) reactions highlight carbohydrate-rich structures, turning them magenta after oxidation and Schiff reagent treatment. Fungal cell walls contain abundant polysaccharides such as chitin and glucans, which react strongly with PAS. The Hotchkiss-McManus modification of this stain is used in histology to visualize fungal elements in fixed tissue sections, making fungi the best target for demonstration with this reaction. Bacteria, nuclei, and spores don’t rely on the same polysaccharide-rich cell-wall features that yield a strong PAS signal in this context, so they are not the primary targets of this technique.

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