Holzer's Method is used to demonstrate which tissue element?

• A. Glial fibers
• B. Neurons
• C. Myelin
• D. Blood vessels

Answer: (A)

Holzer's method is a stain that highlights the delicate network of glial fibers in the CNS. It brings out the astrocytic processes and the glial framework that support and organize the nervous tissue, making these fibrils stand out against the surrounding neurons, myelin, and blood vessels. This makes glial fibers the tissue element demonstrated by this technique.

Neurons would be identified by their cell bodies and distinct neuronal processes with other stains, not by Holzer's method. Myelin is best seen with myelin-specific stains such as Luxol fast blue or osmium, and blood vessels are highlighted by stains that emphasize basement membranes or vascular structures. So Holzer's method uniquely points to the glial fibrous network.