Glycogen containing tissue fixed with Bouin's solution may show resistance to diastase digestion.

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Multiple Choice

Glycogen containing tissue fixed with Bouin's solution may show resistance to diastase digestion.

Explanation:
Glycogen detection by PAS relies on removing glycogen with diastase before staining. Fixatives can alter how well diastase works. Bouin's fixative contains picric acid and formaldehyde, which can chemically modify glycogen or create cross-links that hinder enzyme access. Because of these chemical changes, glycogen in Bouin-fixed tissue may not be completely digested by diastase, leading to persistent PAS staining. Therefore, tissue fixed with Bouin's solution may show resistance to diastase digestion. For reliable diastase testing, using a different fixative (like neutral buffered formalin) or being aware of this Bouin’s effect is important.

Glycogen detection by PAS relies on removing glycogen with diastase before staining. Fixatives can alter how well diastase works. Bouin's fixative contains picric acid and formaldehyde, which can chemically modify glycogen or create cross-links that hinder enzyme access. Because of these chemical changes, glycogen in Bouin-fixed tissue may not be completely digested by diastase, leading to persistent PAS staining. Therefore, tissue fixed with Bouin's solution may show resistance to diastase digestion. For reliable diastase testing, using a different fixative (like neutral buffered formalin) or being aware of this Bouin’s effect is important.

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