For removing formalin pigment, slides should be treated with 10% ammonium hydroxide in 70% alcohol for which time range?

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Multiple Choice

For removing formalin pigment, slides should be treated with 10% ammonium hydroxide in 70% alcohol for which time range?

Explanation:
Removing formalin pigment relies on alkaline bleaching. Treating the slide with 10% ammonium hydroxide in 70% alcohol raises the pH and bleaches the pigment formed during formalin fixation, with alcohol aiding pigment dissolution and removal. The recommended time range of 30 minutes to 3 hours allows enough exposure to remove the pigment without over-bleaching or damaging tissue detail; shorter times may not remove it, while longer times can compromise staining quality. Adjust within this window based on pigment intensity and tissue thickness, then rinse and proceed with staining.

Removing formalin pigment relies on alkaline bleaching. Treating the slide with 10% ammonium hydroxide in 70% alcohol raises the pH and bleaches the pigment formed during formalin fixation, with alcohol aiding pigment dissolution and removal. The recommended time range of 30 minutes to 3 hours allows enough exposure to remove the pigment without over-bleaching or damaging tissue detail; shorter times may not remove it, while longer times can compromise staining quality. Adjust within this window based on pigment intensity and tissue thickness, then rinse and proceed with staining.

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