For delicate tissues such as embryonic tissues, dehydration should start at which alcohol concentration?

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Multiple Choice

For delicate tissues such as embryonic tissues, dehydration should start at which alcohol concentration?

Explanation:
Starting with a low ethanol concentration provides a gentle, gradual removal of water, which helps preserve the morphology of very delicate tissues like embryos. A 30% ethanol step reduces the risk of osmotic shock and shrinkage that can occur if dehydration begins with higher concentrations. After the initial gentle step, you progress through higher concentrations (50%, 70%, 95%, then 100%) to complete dehydration while maintaining tissue integrity. Using a higher starting concentration can pull water out too quickly, distorting fine embryonic structures; the slow ramp helps maintain their architecture for accurate biopsy and embedding.

Starting with a low ethanol concentration provides a gentle, gradual removal of water, which helps preserve the morphology of very delicate tissues like embryos. A 30% ethanol step reduces the risk of osmotic shock and shrinkage that can occur if dehydration begins with higher concentrations. After the initial gentle step, you progress through higher concentrations (50%, 70%, 95%, then 100%) to complete dehydration while maintaining tissue integrity. Using a higher starting concentration can pull water out too quickly, distorting fine embryonic structures; the slow ramp helps maintain their architecture for accurate biopsy and embedding.

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