Excess heat during processing, especially in dehydration and clearing, can lead to removal of bound water resulting in what artifact?

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Multiple Choice

Excess heat during processing, especially in dehydration and clearing, can lead to removal of bound water resulting in what artifact?

Explanation:
Excess heat during processing removes bound water from the tissue, making it shrink and become brittle. This results in cells that appear wrinkled or scalloped as they contract, which is known as crenation. It’s a dehydration artifact caused by over-dehydration and over-clearing. By contrast, microchatter is a cutting artifact from sectioning, autolysis is enzymatic autolysis of tissue after death, and air artifacts come from trapped air during processing or mounting. So the artifact produced by removal of bound water is crenation.

Excess heat during processing removes bound water from the tissue, making it shrink and become brittle. This results in cells that appear wrinkled or scalloped as they contract, which is known as crenation. It’s a dehydration artifact caused by over-dehydration and over-clearing. By contrast, microchatter is a cutting artifact from sectioning, autolysis is enzymatic autolysis of tissue after death, and air artifacts come from trapped air during processing or mounting. So the artifact produced by removal of bound water is crenation.

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