Eosin is differentiated by dehydrating alcohols.

• A. True
• B. False
• C. Only by acid alcohol
• D. Only by water

Answer: (A)

Differentiating eosin with dehydrating alcohols is about fine‑tuning how the pink stain interacts with tissue. After eosin has colored the cytoplasm and other components, passing the slide through graded alcohols helps remove the portion of eosin that is loosely bound. This selective removal reduces background staining and sets a clear, consistent pink contrast against the hematoxylin-stained nuclei. Acid alcohol is primarily used to differentiate hematoxylin, not eosin, and simply using water would rehydrate the tissue and either increase or alter staining rather than selectively differentiate eosin. So, the statement that eosin is differentiated by dehydrating alcohols is correct.