During AB-PAS staining, neutral mucins appear which color?

Prepare for the Histotechnologist Certification Exam with our comprehensive study material. Use flashcards, detailed explanations, and intuitive multiple-choice questions. Boost your test readiness and achieve certification success!

Multiple Choice

During AB-PAS staining, neutral mucins appear which color?

Explanation:
AB-PAS differentiates mucins by using two stains: Alcian blue for acidic mucins and the periodic acid–Schiff (PAS) reaction for neutral mucins. Alcian blue binds to the acidic mucins and makes them blue, while PAS reacts with carbohydrate groups in neutral mucins to produce a magenta/pink color. So neutral mucins appear pink. If both types are present in the same area, their colors can overlap to produce a purple hue, but the distinct color of neutral mucins is pink.

AB-PAS differentiates mucins by using two stains: Alcian blue for acidic mucins and the periodic acid–Schiff (PAS) reaction for neutral mucins. Alcian blue binds to the acidic mucins and makes them blue, while PAS reacts with carbohydrate groups in neutral mucins to produce a magenta/pink color. So neutral mucins appear pink. If both types are present in the same area, their colors can overlap to produce a purple hue, but the distinct color of neutral mucins is pink.

Subscribe

Get the latest from Passetra

You can unsubscribe at any time. Read our privacy policy