Diastase digestion reduces staining intensity of which staining method?

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Multiple Choice

Diastase digestion reduces staining intensity of which staining method?

Explanation:
Diastase digestion targets glycogen, a carbohydrate that gives a strong PAS signal. The PAS reaction works by oxidizing vicinal diols in carbohydrates to aldehydes, which Schiff reagent then stains magenta. Glycogen provides lots of these reactive sites, so it yields intense PAS staining. When tissue is treated with diastase (amylase), glycogen is broken down into smaller sugars and removed, leaving fewer aldehyde groups after oxidation. That drop in substrate makes the magenta color much lighter, so staining intensity decreases. This is why diastase digestion is used before PAS to distinguish glycogen from other PAS-positive substances.

Diastase digestion targets glycogen, a carbohydrate that gives a strong PAS signal. The PAS reaction works by oxidizing vicinal diols in carbohydrates to aldehydes, which Schiff reagent then stains magenta. Glycogen provides lots of these reactive sites, so it yields intense PAS staining. When tissue is treated with diastase (amylase), glycogen is broken down into smaller sugars and removed, leaving fewer aldehyde groups after oxidation. That drop in substrate makes the magenta color much lighter, so staining intensity decreases. This is why diastase digestion is used before PAS to distinguish glycogen from other PAS-positive substances.

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