Before processing decalcified tissue, what is the next step?

• A. Neutralize acid
• B. Rinse with acetone
• C. Embed in paraffin
• D. Stain with H&E

Answer: (A)

Decalcification uses acid to dissolve calcium, so the immediate need after decalcification is to halt that process and remove any residual acid. Neutralizing the acid raises the pH and stops further demineralization, protecting tissue morphology and ensuring proper infiltration during the subsequent dehydration and embedding steps. After neutralizing, the tissue is thoroughly rinsed to remove salts and byproducts, then it proceeds to dehydration, clearing, and embedding. Rinsing with acetone would dehydrate and isn’t how you stop decalcification, and embedding or staining come later in the workflow after processing is complete.