At a staining solution pH between 4.6 and 5.0, eosin combines with which tissue chemical group?

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Multiple Choice

At a staining solution pH between 4.6 and 5.0, eosin combines with which tissue chemical group?

Explanation:
At this staining pH, eosin acts as a negatively charged (acidic) dye and binds to positively charged basic groups in tissue. The amino groups on proteins become protonated to NH3+ in acidic conditions, providing an attractive site for the eosin dye. Therefore, the tissue chemical group eosin associates with is NH3+. Carboxylate (COO−) carries a negative charge and would not attract eosin; SH and OH are not positively charged under these conditions and do not form the same ionic interaction.

At this staining pH, eosin acts as a negatively charged (acidic) dye and binds to positively charged basic groups in tissue. The amino groups on proteins become protonated to NH3+ in acidic conditions, providing an attractive site for the eosin dye. Therefore, the tissue chemical group eosin associates with is NH3+. Carboxylate (COO−) carries a negative charge and would not attract eosin; SH and OH are not positively charged under these conditions and do not form the same ionic interaction.

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