Alcian Blue at pH 2.5 yields which background color?

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Multiple Choice

Alcian Blue at pH 2.5 yields which background color?

Explanation:
Alcian Blue is a positively charged dye that binds to negatively charged acidic mucins. At pH 2.5, it stains both sulfated and carboxylated mucopolysaccharides blue. The rest of the tissue that doesn’t take up the blue is shown against the counterstain, which is typically eosin or another pink/red counterstain, producing a pink to red background. This contrast helps mucins appear blue against a pink/red background. If a different counterstain is used, the exact shade may vary, but pink to red is the common background.

Alcian Blue is a positively charged dye that binds to negatively charged acidic mucins. At pH 2.5, it stains both sulfated and carboxylated mucopolysaccharides blue. The rest of the tissue that doesn’t take up the blue is shown against the counterstain, which is typically eosin or another pink/red counterstain, producing a pink to red background. This contrast helps mucins appear blue against a pink/red background. If a different counterstain is used, the exact shade may vary, but pink to red is the common background.

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