After Schiff reagent, tissues are rinsed in a sulfite solution to remove the excess which substance?

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Multiple Choice

After Schiff reagent, tissues are rinsed in a sulfite solution to remove the excess which substance?

Explanation:
In this staining step, the sulfite rinse is used to remove the unbound Schiff reagent. Schiff reagent starts in a colorless reduced form called leucofuchsin; when it encounters aldehyde groups, it becomes the magenta Schiff color. The sulfite bath bleaches and washes away any remaining leucofuchsin that didn’t react, preventing nonspecific background staining, while the aldehyde-Schiff reaction sites remain magenta. The other dyes listed are unrelated to this step and are not what the sulfite rinse targets.

In this staining step, the sulfite rinse is used to remove the unbound Schiff reagent. Schiff reagent starts in a colorless reduced form called leucofuchsin; when it encounters aldehyde groups, it becomes the magenta Schiff color. The sulfite bath bleaches and washes away any remaining leucofuchsin that didn’t react, preventing nonspecific background staining, while the aldehyde-Schiff reaction sites remain magenta. The other dyes listed are unrelated to this step and are not what the sulfite rinse targets.

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