After fixation with Clark solution, tissue must be washed with which concentration of alcohol as the first step in processing?

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Multiple Choice

After fixation with Clark solution, tissue must be washed with which concentration of alcohol as the first step in processing?

Explanation:
The main idea here is removing all water from the tissue to prepare it for paraffin infiltration. After fixation with Clark solution, the tissue may still contain water and fixative residues. Using absolute alcohol (95–100%) as the first dehydration step quickly replaces that water and helps wash away fixative remnants, giving a clean start for subsequent dehydration and embedding. Lower concentrations (like 70%, 50%, or 25%) would leave more water behind, risking incomplete dehydration and artifacts during processing. So starting with 95–100% alcohol is the best choice.

The main idea here is removing all water from the tissue to prepare it for paraffin infiltration. After fixation with Clark solution, the tissue may still contain water and fixative residues. Using absolute alcohol (95–100%) as the first dehydration step quickly replaces that water and helps wash away fixative remnants, giving a clean start for subsequent dehydration and embedding. Lower concentrations (like 70%, 50%, or 25%) would leave more water behind, risking incomplete dehydration and artifacts during processing. So starting with 95–100% alcohol is the best choice.

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