A Ziehl-Neelsen control slide is typically prepared from which tissue?

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Multiple Choice

A Ziehl-Neelsen control slide is typically prepared from which tissue?

Explanation:
Ziehl-Neelsen staining is used to detect acid-fast bacilli, such as Mycobacterium tuberculosis, which retain carbol fuchsin after acid-alcohol decolorization due to their waxy cell wall. A positive control should come from tissue that reliably contains these organisms so you can verify that the stain, decolorization, and counterstain steps are working. Known TB-infected lung tissue provides visible red acid-fast bacilli, confirming the procedure. Normal liver, skeletal muscle, or brain tissue typically lack acid-fast organisms and would not demonstrate the expected staining, so they are not suitable controls.

Ziehl-Neelsen staining is used to detect acid-fast bacilli, such as Mycobacterium tuberculosis, which retain carbol fuchsin after acid-alcohol decolorization due to their waxy cell wall. A positive control should come from tissue that reliably contains these organisms so you can verify that the stain, decolorization, and counterstain steps are working. Known TB-infected lung tissue provides visible red acid-fast bacilli, confirming the procedure. Normal liver, skeletal muscle, or brain tissue typically lack acid-fast organisms and would not demonstrate the expected staining, so they are not suitable controls.

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